Anti-Thymidylate Synthase (TS) (BSA & azide free), clone 3F359

Thymidylate Synthase (TS)1 (EC 2.1.1.45) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal,4,5 gastric,5 head and neck,6 and breast7 carcinomas. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: Use for coating., Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate , Western Blot: 1-2ug/ml for 2hrs at RT , Optimal dilutions to be determined by the researcher. Positive Control: 5-FU-resistant cell cancer lines or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.