Anti-TEM8 (Tumor Endothelial Marker 8), clone 4H261

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml , Expected size is ~80kD on HUVEC cells and is an 80kD and 85kD double in human colon cell lysates. The size difference is thought to be due to glycosylation as a 70kD band was seen when cell extracts were treated with a glycosidase cocktail., Immunohistochemistry (paraffin): 25ug/ml , Optimal dilutions to be determined by the researcher. Positive Control: TEM8 transfected cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.