Anti-TCR, gamma, delta (T Cell Receptor), Phycoerythrin conjugated, clone 5K81

This antibody precipitates a heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35kD and 32kD from antibody precipitates. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gd T cells. The frequency of TCRgd cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age. The majority of TCRgd+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgd+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gd T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gd T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions. Applications:  Flow cytometry 1-3,  Immunohistochemistry (acetone-fixed, frozen sections) 5,  Immunoprecipitation 1-3,  In ovo depletion of gd T cells 6,7, Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.