Anti-STAT3 (STAT3, APRF, signal transducer and activator of transcription 3 (acute-phase response

Domain Information: SH2 domain, STAT protein, DNA binding domain, STAT protein, all-alpha domain, STAT protein, protein interaction domain. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA (Peptide): 1:128,000 (antibody detection limit dilution), Western Blot: 1-3ug/ml. A band at ~90kD is observed in NIH3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour, detection by ECL. Predicted MW of 88kD according to NP_003141.2., Immunohistochemistry: 10ug/ml (overnight incubation). Stains mouse thymic medulla and not the cortex., Optimal dilutions to be determined by the researcher. Peptide Blocking: S7971-01M-P, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.