Anti-STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase

The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and proliferation), depending upon the cell type. STATs are activated by ligand binding to cytokine receptors and other types of receptors, those receptors associating with Janus kinase (JAK) family members that phosphorylate STAT protein, causing it to translocate to the nucleus. In the nucleus, phosphorylated STAT protein binds to DNA and regulates the transcription of particular genes. Phosphorylation on tyrosine 705 by JAK1&2 is essential for STAT3 dimer formation, nuclear translocation and DNA binding activity. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Positive Control: 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.