Anti-STAT1 (Signal Transducer and Activator of Transcription 1), clone 5J111

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT 1 and 2) involved in IFN-a and IFN-g signal transduction. Since their initial identification, 5 more STAT proteins have been discovered (STAT 3, 4, 5a, 5b and 6). STATs undergo tyrosine phosphorylations in response to growth factor or cytokine signaling (in some cases mediated by JAK kinases [Janus Kinases 1, 2 and 3]), resulting in dimerization and translocation of STAT proteins to the nucleus. Phosphorylation at serine residues in certain STATs (STAT 1a, 3, 4 and 5) has also been reported, and appears to be required for the maximal activation of these proteins. This antibody recognizes proteins of ~91 and ~84kD, identified as STAT 1a and b. STAT 1 participates in a signaling pathway which is initiated by IFN-g. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human Jurkat and HeLa cells, mouse L929 cells and rat thymocytes. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.