Anti-SOAT-1/ACAT-1

Sterol O-acyltransferase 1 (SOAT-1), also known as acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1), is an enzyme encoded by ACAT1 in humans that catalyzes the intracellular formation of cholesterol esters from cholesterol and long-chain fatty acyl-coenzyme A. It is ubiquitously expressed and localized to the rough endoplasmic reticulum where it preferentially utilizes oleic acid (Cay-90260, Cay-24659) or palmitic acid (Cay-10006627) as fatty acid substrates for the synthesis of cholesterol esters, which are stored intracellularly or packaged into chylomicrons or VLDL and secreted into the blood stream. SOAT-1/ACAT-1 protein levels are increased in macrophages under various pathological conditions, including atherosclerosis. SOAT-1/ACAT-1 activity is increased by cholesterol in vitro, and ACAT1 expression is increased by stimulation with the pro-inflammatory cytokines TNF-alpha or IFN-gamma in isolated human and THP-1 monocytes, respectively. ACAT1 silencing in human H4 neuroglioma cells overexpressing amyloid-beta precursor protein (APP) reduces secretion of soluble amyloid-beta (Abeta) and Abeta42 (Cay-20574). Genome-wide deletion of Acat1 reduces macrophage infiltration and neutral lipid deposition in atherosclerotic aortic lesions and decreases serum total cholesterol levels, but increases brain cholesterol deposition, in ApoE-/- mice fed a Western diet. Cayman's SOAT-1/ACAT-1 Polyclonal Antibody can be used for immunoflouresence (IF) immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the N-terminus to detect full-length SOAT-1/ACAT-1 at approximately 65 kDa from human, mouse, porcine, and rat samples.Synonyms: Acyl-coenzyme A: Cholesterol Acyltransferase-1, Cholesterol Acyltransferase 1, Sterol O-Acyltransferase 1. Immunogen: Synthetic peptide from the N-terminal region of human SOAT-1/ACAT-1. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: IF, IHC, WB. UniProt Accession #: P35610. Origin: Animal/Rabbit.