Anti-SLP-76 (LCP2, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)

SLP-76 is a 533aa, 76kD tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLCgamma, ITK, and , ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. , Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, , respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.