Anti-SIRT3 (Sirtuin 3, SIR2L3, SIR2-like protein 3)

SIRT3 (SIR2­like protein 3) is a 28­30kD, NAD+­dependent class III member of the sirtuin protein family. It is widely expressed, and found apparently in both the nucleus, where it deacetylates histones, and in the mitochondrial matrix, where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates, including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100aa, followed by a sertuin­type deacetylase domain (aa126­382) that contains an NAD binding segment (aa145­165). Although uncertainty exists relative to its intracellular location, it would appear that SIRT3 is initially synthesized as a 42­44kD "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28­30kD short form. There is one potential alternative start site at Met143. Over aa101­399, human and mouse SIRT3 share 84% aa sequence identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.