Anti-Sialoprotein, Bone (BSP), clone 2Q556

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, Immunohistochemistry: Paraffin embedded sections, Optimal dilutions to be determined by the researcher. Positive Control: Human osteoblasts or osteocytes, Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.-