Anti-SFRP4 (Secreted Frizzled-related Protein 4, sFRP-4, Frizzled Protein, Human Endometrium, FrpHE)

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Peptide): 1:32,000, Western Blot: 1-3ug/ml detects a band at ~40kD in human uterus lysates, Immunohistochemistry (Paraffin): 2-4ug/ml using paraffin embedded human uterus shows grandular staining in the apical cytoplasm of the endometrial gland, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.