Anti-Retinoblastoma (RB) Protein, Cleaved, clone 4H246

The retinoblastoma gene product (RB) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, RB has been shown to play a role in apoptosis. During apoptosis, RB is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves RB into 48 and 68kD fragments (2-4). Cleavage of RB would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml , Whole cell lysate from camptothecin induced apoptotic HL60 cell can be used as positive control and a band of approx. size of 68 kD should be detected. ., Optimal dilutions to be determined by the researcher, Positive Control: Camptothecin treated HL60. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.