Anti-Retinoblastoma (Rb) (BSA & azide free), clone 3F328

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. MW of Antigen: 105kD , Epitope: aa703-722 , Positive Control: Ls174T, HT29, HUVEC cells. Normal colon and carcinoma. Breast carcinoma. Cellular Localization: Nuclear Applications and Recommended Dilutions: , Flow Cytometry , Immunofluorescence1 , Immunoprecipitation (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate) , Western Blotting (Ab 1-2ug/ml for 2hrs at RT) , Immunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 , for 10-20 min followed by cooling at RT for 20 min.) [EDTA is better than citrate for epitope unmasking] The optimal dilution for a specific application should be determined by the investigator. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.