Anti-Renal Cell Carcinoma Marker, Aminopeptidase A

Monoclonal antibody (MAb) RC38 recognizes a human renal antigen of 160 kD recently identified as human aminopeptidase A (APA, EC 3.4.11.7). This ectoenzyme is able to hydrolyse selectively N-terminal glutamyl and aspartyl residues from oligopeptides. By enzyme histochemistry, APA activity has also been localized in the microvessels of all organs in animals and man. A study was done to investigate the distribution of human APA as recognized by MAb RC38 in the microvasculature of normal human tissues and pathological conditions associated with neovascularization. Unexpectedly, in normal tissues vascular staining with MAb RC38 was generally weak and often absent, while in tumors, granulation tissue, and chronic synovitis, marked microvascular staining was demonstrated. By immuno-electron microscopy, the antigen was found on the cell membrane of activated pericytes and their processes in the tumor vasculature. RC38 expression could not be detected on cultured human endothelial cells or pericytes. These observations suggest that pericyte expression of a subtype of APA (as recognized by MAb RC38) is markedly enhanced in the vasculature of tumors and wound healing tissue as compared with normal resting tissues. This provides further evidence of the altered state of pericytes in these conditions. Pericyte APA may be involved in the metabolism of biologically active oligopeptides during neovascularization, supporting a regulatory role of pericytes in this process. In addition, MAb RC38 may be useful as a marker of pericyte activation in tissue sections. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:10 using the Indirect Immunoperoxidase method. Dilute in PBS., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;30.06.2011;03.03.2021;;;ml;R1450-03J;1;384, 385;;IHC;-20;+4;;+4;;;;;human;;Renal Cell Carcinoma ;Mab;No;RC38;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1450-03J;;;;;;;;;;purified;32160702;0 R1460-05.100;R1460-05.100;Anti-Renin, Pro/Mature;USBio;706;0;Renin is secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin. The 406aa precursor includes a signal peptide (aa 1-23), a propeptide (aa 24-66), and a mature chain (aa 67-406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat. Applications: Suitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml, Western Blot: 1ug/ml under non-reducing conditions only, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;30.06.2011;03.03.2021;Anti-REN, Anti-Renin, EC=3.4.23.15, Anti-Angiotensinogenase;;µg;R1460-05;100;384, 385;;ELISA*IP*WB;-20;+4;;+4;;;;;human;;NS0-derived recombinant human Renin pro form (aa24-406) Accession # P00797;Mab;No;7H238;Mouse;GeneID 5972;P00797;K01380;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1460-05;;;;;;;;;;affinity purified;32160702;0 R1460-40.100;R1460-40.100;Anti-Renin-1 (Angiotensinogenase, Kidney Renin);USBio;748;0;Mouse Renin is a secreted, 42-47kD glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381aa proform (aa22-402). In the kidney, pro-Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-Renin shares 70% and 85% aa sequence identity with human and rat pro-Renin, respectively. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml, Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant mouse Renin, Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse kidney, ELISA (Capture): 0.2-0.8ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;30.06.2011;03.03.2021;Anti-Ren, Anti-Ren1, Anti-Renin-1, EC=3.4.23.15, Anti-Kidney renin, Anti-Angiotensinogenase;;µg;R1460-40;100;384, 385;;ELISA*IHC*IP*WB;-20;+4;;+4;;;;;mouse;;NS0-derived recombinant mouse Renin-1 (aa22-402) Accession # P06281;Pab;No;;Goat;GeneID 19701;P06281;K01380;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1460-40;;;;;;;;;;affinity purified;32160702;0 R1600-01.100;R1600-01.100;Anti-Respiratory Syncytial Virus, fusion protein (RSV), clone 2Q2054;USBio;549;0;RSV belongs to the family Paramyxoviridae and the genus Pneumovirus. It is an enveloped pleomorphic virus ranging from 150-300nm in diameter with a single-stranded RNA genome. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;02.02.2004;03.03.2021;;;µl;R1600-01;100;384, 385;;ELISA;-20;+4;;-20;;;;;;;;Mab;No;2Q2054;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1600-01;;;;;;;;;;ascites;32160702;0 R1701-01.100;R1701-01.100;Anti-Retinol Binding Protein (RBP);USBio;546;0;RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Strongest reactions seen in liver tissue., Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;30.06.2011;03.03.2021;;;µg;R1701-01;100;384, 385;;ELISA*IHC;-20;+4;;+4;;;;;goat, human, monkey, mouse, rat;;Human retinol binding protein (RBP) purified from plasma;Mab;No;G4E4;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1701-01;;;;;;;;;;purified;32160702;0 R1701-35C.100;R1701-35C.100;Anti-Retinol Binding Protein 4 (RBP4, PRBP, PRO2222);USBio;678;0;RBP4, also known as plasma retinol-binding protein, is a member of the lipocalin superfamily that solubilizes and stabilizes retinol (vitamin A) for transport to the peripheral tissues. RBP4 shows retinol-dependent secretion by the liver, and its deficiency causes night blindness. In plasma, the 183 aa RBP4-retinol complexes with transthyretin (TTR), previously known as thyroxine-binding prealbumin, which prevents loss during glomerular filtration. Human and mouse RBP4 share 86% aa identity. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. , Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml, Flow Cytometry): 25ug/ml using HeG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeablized with methanol, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;30.06.2011;03.03.2021;;;µg;R1701-35C;100;384, 385;;ELISA*FC*WB;-20;+4;;-20;;;;;human;;NS0-derived rhRBP4.;Mab;No;7H193;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1701-35C;;;;;;;;;;affinity purified;32160702;0 R1701-35K.250;R1701-35K.250;Anti-Retinol Binding Protein 4 (RBP4, PRBP, PRO2222);USBio;416;0;RBP4, also known as plasma retinol-binding protein, is a member of the lipocalin superfamily that solubilizes and stabilizes retinol (vitamin A) for transport to the peripheral tissues. RBP4 shows retinol-dependent secretion by the liver, and its deficiency causes night blindness. In plasma, the 183 aa RBP4-retinol complexes with transthyretin (TTR), previously known as thyroxine-binding prealbumin, which prevents loss during glomerular filtration. Human and mouse RBP4 share 86% aa identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;24.06.2009;03.03.2021;;;µg;R1701-35K;250;384, 385;;ELISA*WB;-20;+4;-20°C Glycerol;+4;;Protein A affinity chromatography.;;;human;;Synthetic peptide corresponding to human recombinant Retinol Binding Protein 4.;Mab;No;9F63;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1701-35K;;;;;;;;;;affinity purified;32160702;0 R1992-45.100;R1992-45.100;Anti-Rex-1 (Reduced Expression Protein 1, Zinc Finger Protein 42, ZFP42);USBio;678;0;Human Rex-1 (also Zfp-42) is a 35kD zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa214-307. Over this region, human and mouse show modest homology with 47% amino acid identity. Applications: Suitable for use in Immunocytochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilution: Immunocytochemistry: 10ug/ml was used to detect REx-1 on NTERA2 cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted R1992-45 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA., Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane and under non-reducing and reducing conditions. ECL detection will increase sensitivity by 5 to 50 fold., Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;31.01.2012;03.03.2021;;;µg;R1992-45;100;384, 385;;ELISA*ICC*WB;-20;+4;;+4;;;;;human;;Recombinant corresponding to aa1-150 of human Rex-1 expressed in E. coli (NP_777560).;Mab;No;7H157;Rat;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1992-45;;;;;;;;;;affinity purified;32160702;0 R1998-13.200;R1998-13.200;Anti-RhoE (Rho-related GTP Binding Protein, Rnd3), clone 5H35;USBio;613;0;The Rho GTPase family member RhoE regulates actin filaments partly by binding to and inhibiting ROCK I, a serine/threonine kinase that induces actomyosin contractility. Here, we show that ROCK I can phosphorylate multiple residues on RhoE in vitro. In cells, ROCK I-phosphorylated RhoE localizes in the cytosol, whereas unphosphorylated RhoE is primarily associated with membranes. Phosphorylation has no effect on RhoE binding to ROCK I, but instead increases RhoE protein stability. Using phospho-specific antibodies, we show that ROCK phosphorylates endogenous RhoE at serine 11 upon cell stimulation with platelet-derived growth factor, and that this phosphorylation requires an active protein kinase C signalling pathway. In addition, we demonstrate that phosphorylation of RhoE correlates with its activity in inducing stress fibre disruption and inhibiting Ras-induced transformation. This is the first demonstration of an endogenous Rho family member being phosphorylated in vivo and indicates that phosphorylation is an important mechanism to control the stability and function of this GTPase-deficient Rho protein. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells., Immunocytochemistry: 1:50. Has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100., Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: F4209: Fibroblast Cell, 3T3/A3. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;13.01.2006;03.03.2021;;;µl;R1998-13;200;384, 385;;ICC*WB;-20;+4;frozen cool packs;+4;;;;;human, mouse;;;Mab;No;5H35;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R1998-13;;;;;;;;;;ascites;32160702;0 R2027-90.100;R2027-90.100;Anti-Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2);USBio;1239;0;The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle- specific proteins. On average, snoRNPs contain about 6-10 protein subunits. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Under less stringent washing conditions, a weaker band of ~60kD is also detected., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.;0;3 - 19;03.07.2012;03.03.2021;Anti-POP7, Anti-hPOP7, Anti-RPP20, EC=3.1.26.5, Anti-RNaseP protein p20, Anti-Ribonuclease P protein subunit p20, Anti-Ribonucleases P/MRP protein subunit POP7 homolog;;µg;R2027-90;100;384, 385;;ELISA*WB;-20;+4;;+4;;;;;human;;Recombinant GST-hRPP20 (Accession #AAC24113, expression vector pGEX-2T), expressed in E.coli.;Mab;No;5BH5;Mouse;GeneID 10248;O75817;K14527;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R2027-90;;;;;;;;;;affinity purified;32160702;0 R2028-75B.100;R2028-75B.100;Anti-Ribonucleoprotein Particles (RNP);USBio;546;0;Applications: , Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Cells must be made permeable. Incubation with R2028-75B should be carried out at 37ºC., Immunohistochemistry: Frozen and paraffin sections, Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;30.06.2011;03.03.2021;;;µg;R2028-75B;100;384, 385;;FC*IHC;-20;+4;;+4;;;;;;;Isolated nuclei.;Mab;No;58-15;Mouse;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R2028-75B;;;;;;;;;;purified;32160702;0 R2031-51C.1;R2031-51C.1;Anti-Ricinus communis RCA60, RCA120 (Agglutinin I, Agglutinin II, Castor Bean);USBio;508;0;These antibodies to lectins are produced by hyperimmunizing goats with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns. Each anti-lectin is supplied lyophilized in buffered saline. These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells, or following lectin transport in neuronal tracing. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1-10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.;0;3 - 19;14.08.2012;03.03.2021;;;mg;R2031-51C;1;384, 385;;ELISA;-20;+4;;+4;;;;;;;;Pab;No;;Goat;;;;;https://www.biomol.com/datasheets/usbio/datasheet.php?item=R2031-51C;;;;;;;;;;affinity purified;32160702;0 R2031-52B.500;R2031-52B.500;Anti-Ricin communis RCA60 (RCA II, castor bean), clone 5E780;USBio;533;0;Ricin is a protein toxin that is readily produced from castor beans (Lectin Ricinus communis) which inhibits protein synthesis. Red blood cells agglutinate due to the presence of RCA (Ricinus communis agglutinin) a potent cytotoxin but a weak hemagglutinin. Ricin and RCA are synthesized in the endosperm cells of maturing seeds, and are stored in an organelle called the ""protein body"", a vacuolar compartment. When the mature seed germinates, the toxins are destroyed by hydrolysis within a few days. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer."