Anti-Renal Cell Carcinoma (CD205, CLEC13B, DEC-205, gp200 MR6, gp200-MR6, Lymphocyte Antigen 75, LY-

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains. Applications: Suitable for ELISA and Flow Cytometry. Other Applications not tested. Recommended Dilutions: Flow Cytometry (Blood derived monocytes): 25ug/ml. Add 10ul to 1-2.5x10e5 celss in a total rection not exceeding 200ul., ELISA: 0.5-1ug/ml. The detection limit is ~1ng/ml for recombinant human DEC-205., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.