Anti-Rb2, p130 (Tumor Suppression Protein)

Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105) Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain.". Both proteins play a fundamental role in growth control. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:5000-1:20,000, Western Blot: 1:500-1:2000, Immunohistochemistry (Paraffiin embedded): 1:200-1:1000 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.