Anti-RASSF1A, clone 5G1

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence (IF-IC). Other applications not tested. Recommended Dilutions: Western Blot:1:500-1:2000, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RASSF1A protein. Source: Ascites, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.