Anti-RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted, CCL5)

RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Applications: Suitable for use in ELISA, Western Blot, Neutralization. Recommended Dilution: Neutralization of Feline CCL5 Bioactivity: The exact concentration of antibody required to neutralize rfeRANTES activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-feline RANTES antibody was determined to be approximately 0.5-2.5ug/ml in the presence of 80ng/mL of rfeRANTES, using transfected BaF/3 cells in a chemotaxis assay. The specific conditions are described in the figure legends. , Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 1ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 0.5ng/well. In this format, this antibody shows less than 5% cross-reactivity with rcrRANTES, rhRANTES, and rmRANTES. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.