Anti-RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted, CCL5), Biotin conjugated

RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.15-0.30ug/ml, Western Blot: 0.1-0.2ug/ml, Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: S7973-95: Streptavidin (TRITC), S7973-92F2: Streptavidin (PE), Matched Pair: Detection: R1100-01E, Capture: R1100-01C, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.