Anti-RANKL (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand,

CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE) is a member of the tumor necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases. It is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses. Blocks ligand binding. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:20, 10ul labels 10e6 cells in 100ul, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.