Anti-Ran (Androgen Receptor-associated Protein 24, ARA24, Gsp1, GTP Binding Nuclear Protein RAN, GTP

Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport. During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation. In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with 1X TBS, 5% BSA. 0.1% Tween-20 at 4ºC with gentle shaking overnight., Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.