Anti-Rad51, clone 6A174

Rad51 is a structural and functional homolog of Escherichia coli RecA, which is required for genetic recombination, DNA repair, and cellular proliferation. This 43kD protein plays an important role in the repair of DNA double-strand breaks caused by ionizing radiation or from the breakdown of stalled replication forks. It forms helical nucleoprotein filaments in which the DNA lies extended and underwound. Filaments form preferentially on tailed duplex DNA substrates at the chromosomal break sites. Rad51 expression is increased in transformed cells and in cancer cell lines. Rad51 is overexpressed in breast carcinomas. Aberrant overexpression of Rad51 is directly correlated with radiation resistance and chemotherapeutic drug resistance. This aberrant expression of Rad51 is also related to enhanced recombination and genomic instabilities in breast carcinoma cells. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml, Immunoprecipitation: 10ug/IP Reaction, Optimal dilutions to be determined by the researcher, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.