Anti-Rac2 (RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein R

Applications:, Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 (detection limit), Western Blot: 1-3ug/ml. Approx 22kD band observed in lysates of Human T cell line MOLT4 (calculated MW of 21.4kD according to NP_002863.1). Staining (2ug/ml) of MOLT-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence., Immunohistochemistry: 0.3ug/ml in paraffin embedded human tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centers the majority of cells are negative. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer., ,