Anti-Protein Kinase D3 (PKD3, PRKD3, EPK2, Protein Kinase C nu Type, PKCnu, PKC-nu, nPKC nu, nPKC-nu

PKCv, also known as PKD3, is a member of the protein kinase C (PKC) family of erine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation. PKCv is composed of 890aa residues and has 77.3% similarity to human PKCu (PKCu) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCu) (1). The PKCv mRNA is ubiquitously expressed in various tissues. PKCv has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCv is translocated to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCv is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induce a rapid activation of PKCv by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCv. PKCv is present both in the nucleus and cytoplasm and this distribution of PKCv results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKCv that is PKC-dependent. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.