Anti-Protein Kinase C, gamma (PKCg)

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. It is involved in diverse cellular signaling pathways. Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells., PKC family is divided into three groups based on different enzymes' cofactor requirements. They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively. Conventional (c) PKC isoforms consisting alpha, betaI, betaII and gamma require diacylglycerol (DAG), Ca2+, and phospholipid for activation. Novel (n)PKC isoforms consisting delta, epsilon, eta (also known as PKC-L), theta and µ (the mouse homolog of human PKCµ, known as PKD) require DAG but not Ca2+ for activation. Atypical (a)PKC isoforms, consisting zeta, iota and lambda (the mouse homolog of human PKC iota) require neither Ca2+ nor DAG. Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCnu. PKCgamma (PKC gamma) plays a key role in neuronal signal transduction and regulating intercellular communication. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.