Anti-Prostaglandin Dehydrogenase 1 (HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD

15-PGDH (15-hydroxyprostaglandin dehydrogenase NAD-linked, also HPGD) is a 28-29kD member of the short-chain non-metalloenzyme family of dehydrogenases. It is widely expressed, and antagonizes COX-2 by catalyzing the oxidation of 15-OH prostaglandin to metabolically inactive 15-keto metabolites. Human 15-PGDH is 266aa in length. A Tyr at position #151 serves as a proton acceptor, and a Ser at #138 binds substrate. 15-PGDH functions as a nondisulfide-linked homodimer. There is one alternate splice form that shows a 12aa substitution for aa167-266. Full-length human 15-PGDH shares 88% aa identity with mouse 15-PGDH. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.