Anti-Pro-Brain-Derived Neurotrophic Factor (Pro-BDNF)

BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247aa. Cleavage of an 18aa signal sequence produces an ~35kD Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14kD BDNF. The propeptides of human and mouse BDNF share 93aa sequence identity. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml using human brain (cerebellum) tissue, Immunohistochemistry: 8-25ug/ml. Use immersion fixed paraffin-embedded sections of human brain (spinal cord) subjected to antigen retrieval., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.