Anti-Pro-Brain-Derived Neurotrophic Factor (Pro-BDNF)

BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor (NTR). The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active BDNF. The propeptides of human and mouse BDNF share 93% sequence identity. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml, Immunohistochemistry: 25ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.