Anti-Pregnancy Associated Plasma Protein A (PAPP-A, Pappalysin 1, PAPA, PAPPA, PAPP-A, PAPPA1, Aspec

PAPP-A was initially identified as a high molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two 200kD PAPP-A subunits and two 50-90kD subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down's syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down's syndrome. PAPP-A has also been shown to exist in a homodimeric, 400kD form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes. Applications: Detection of human PAPP-A antigen in ELISA, Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched Pair for the detection of PAPP-A in blood of AMI patients and patients with unstable angina (Capture-Detection): P6000-84 - P6000-86, P6000-84 - P6000-87, P6000-82A - P6000-80A , Matched Pair for the detection of PAPP-A in pregnancy serum (Capture - Detection): P6000-84 - P6000-86, P6000-84 - P6000-87, P6000-85 - P6000-86, P6000-82A - P6000-80A, P6000-82A - P6000-81A, P6000-81A - P6000-82A, Hybridoma: Derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP complex. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.