Anti-PRAS40 (Proline-Rich AKT Substrate)

Anti-PRAS40 (Proline-Rich AKT Substrate)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P5640-01.100 100 µl - -

1 - 19 business days

496.00€
 
Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which... more
Product information "Anti-PRAS40 (Proline-Rich AKT Substrate)"
Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40kD, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulindeprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: P5640-01

Properties

Application: WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence of human PRAS40.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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