Anti-PLTP (Plasma Phospholipid Transfer Protein)

PLTP (phospholipid transfer protein) is a 65-81kD, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476aa in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain. There is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions, one shows a one aa (Lys) substitution for aa110-162, and a second shows a four aa substitution for the N-terminal 35aa. Over aa18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml. The detection limit for rhPLTP is ~0.3ng/ml., Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.