Anti-Phosphatase, Alkaline, placental (PLAP), clone 3H2834

Human placental alkaline phosphatase is also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535aa ~67kD GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982) and in ovarian carcinoma (Tholander et al. 1990). P4071-16A has been widely used in immunolocalization studies of germ cell tumors and may also be used in ELISA based assays for the measurement of soluble PLAP. Applications: Suitable for use in Immunohistochemistry, ELISA, and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ug/ml. 10ul labels 10e6 cells in 100ul. , Immunohistochemistry: Frozen sections. Reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation is recommended. Not suitable for use on paraffin sections., Optimal dilutions to be determined by the researcher. Recommended Immunohistology Positive Control: Placenta, seminoma, Recommended Negative Control: , I1904-78R: IgG1 Murine Negative Control, Recommended Secondary Antibodies: , I1904-65V: IgG, F(ab')2 (FITC) Pab RbxMo, I1904-66A: IgG, F(ab')2, X-Adsorbed (FITC) Pab RbxMo, I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo, I1904-66C: IgG, F(ab')2, X-Adsorbed (HRP) Pab RbxMo, Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.