Anti-Phosphacan (Receptor Tyrosine Phosphatase beta), clone 2Q134

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia. It inds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM. It does not bind to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. , Recommended Dilutions: Immunocytochemistry: 1:10 , Immunohistochemistry (4% Paraformaldehyde fixed): 1:1000 Western Blot: Recognizes bands at 250, 190 and 180kD from embryonic rat brain extracts., Optimal dilutions to be determined by the researcher. Positive Control: Embryonic or early post-natal rat brain, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.