Anti-PDGF-Receptor beta (Platelet Derived Growth Factor Receptor Type B), clone

Applications: , Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: A 1:1000 to 1:5000 Detected PDGFRb in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells., Western Blot Analysis: Lysate from NIH/3T3 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with P4258-20 PDGFRb (1:3000 dilution) Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system., Immunoprecipitation: 4ug Immunoprecipitated PDGFRb from 500µg of 3T3/A31 RIPA lysate., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.