Anti-Parvalbumin, clone 3H43

Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent., Western Blot: 1:2000-5000 (ECL). MW: ~12kD., Immunohistochemistry & Immunofluorescence: 1:500-1:1000, Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.