Anti-PARP, cleavage site (Asp214) (Poly ADP-Ribose Polymerase)

PARP, a 116kD nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress. This protein can be cleaved by many ICElike caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP's N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD). PARP is important for cells to maintain their viability, cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% non-fat milk, 0.1% Tween-20 at 4°C with gentle shaking overnight., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.