Anti-Paired Immunoglobulin-Like Type 2 Receptor alpha (PILRa, PILR alpha)

PILR-a (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50kD type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-a acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-a is 284aa in length. It contains one V-type Ig-like domain in its extracellular region (aa32-150), and two ITIMs in its cytoplasmic domain (aa267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa264-303, while others are soluble, and show a deletion of aa152-224 that may be coupled to the 35aa substitution noted above, or simply exhibit a 24aa substitution for aa152-303. Over aa20-196, human PILR-a shares only 42aa identity with mouse PILR-a, and 89aa identity with human PILR-b. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.