Anti-p53R2, NT (p53 Ribonucleotide Reductase)

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry (paraffin): 1ug/ml, Immunofluorescence: 20ug/ml, Optimal dilutions to be determined by the researcher. Blocking Peptide: P1001-32V1, Positive Control: A431 Cell Lysate, 3T3 Cell Lysate and Human Lung Tissue Lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.