Anti-p53 Binding Protein 1 (53BP1, p202), clone 5H36

53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. Small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrated that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. Applications: Suitable for use in Western Blot and Immunoprecipitation(1). Other applications not tested. Recommended Dilution: Western Blot: 1:5000 -1:50,000 detected 53BP1 in whole cell lysates from HeLa cells., Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1905-28 IgM (HRP) Pab GtxMo, I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo, I1905-28R IgM (mu chain specific) (HRP) Pab GtxMo, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.