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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
P1001-24J.100 | 100 µg | - | - |
3 - 19 business days* |
675.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex... more
Product information "Anti-p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1 (47kDa, chronic granulomatous dis"
Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1.5ug/ml, ~48kD band observed in lysates of cell line U937 and of mouse Thymus, and 50kD observed in porcine Spleen lysates, Peptide ELISA: 1:20,000, Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P1001-24J-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Keywords: | Anti-NCF-1, Anti-NCF-47K, Anti-p47-phox, Anti-Nox organizer 2, Anti-Nox-organizing protein 2, Anti-Neutrophil cytosol factor 1, Anti-47 kDa neutrophil oxidase factor, Anti-Neutrophil NADPH oxidase factor 1, Anti-SH3 and PX domain-containing protein 1A |
Supplier: | United States Biological |
Supplier-Nr: | P1001-24J |
Properties
Application: | ELISA, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Goat |
Species reactivity: | human, mouse, swine |
Immunogen: | CSESTKRKLASAV, C Terminus |
Format: | Affinity Purified |
Database Information
KEGG ID : | K08011 | Matching products |
UniProt ID : | P14598 | Matching products |
Gene ID | GeneID 653361 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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