Anti-Obestatin (Appetite-regulating Hormone, Growth Hormone Secretagogue, Growth Hormone-releasing P

Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses. Recently, a new member of the ghrelin-motilin family was identified by using bioinformatic predictions about enzyme cleavage of the prepropeptide of ghrelin. The newly identified 23aa, ghrelin-associated peptide was named obestatin. Although both peptides originate from the same precursor prepropeptide, they have opposing physiological roles. Obestatin exerts anorexigenic effects by decreasing food intake, gastric emptying, jejunal motility, and weight gain by binding to GPR39 orphan receptor, while ghrelin exerts opposing orexigenic effects by binding to growth hormone secretagogue receptor. , Obestatin: Human, mouse, rat 23aa each - 2.6kD, mouse Chromosome: 6E3. Mainly expressed in the gastrointestinal tract with higher levels in the stomach, medium levels in the duodenum, jejunum, ileum and colon. Low expression in the testis and brain. Not detected in the salivary gland, pancreas, liver and lung. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000, Western Blot: 1-10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.