Anti-Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase)

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) hydrolyzes both 3'-5'- phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Applications: Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition. Other applications have not been tested. , Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.