Anti-NP-5 (Alpha Defensin 5, Defensin 5, DEF5, Defensin alpha 5, Defensin alpha 5 Paneth Cell-specif

Human neutrophil alpha-defensins (also designated HNPs) are small, cationic, cysteine-rich antimicrobial proteins that play important roles in innate immunity against infectious microbes such as bacteria, fungi and enveloped viruses. Alpha-defensins are synthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7. In the mature form, NP-5 is cleaved at residue 63 (alanine). Paneth cells in small intestinal crypts secrete the alpha-defensins, which are also termed cryptidins in mice. Alpha-defensins 5 and 6 probably contribute to innate defense of the GI mucosal surface by protecting against microbial invasion in states of intestinal inflammation. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot. Dot Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 5-10ug/ml, Immunohistochemistry (Frozen): 5-10ug/ml, Western Blot: 1:1000, Immunofluorescence (IC): 1:200-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.