Anti-NOL3 (Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein)

NOL3(Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is , encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic , sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP , and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates , the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller , transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when , coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor , necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits , the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC , interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with , CASP1, CASP3, or CASP9. UniProt Number: O60936, Gene ID: NOL3, Applications: Suitable for use in Western Blot. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.