Anti-NF-kappaB (p65) NLS

NF-kappaB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-kappaB complex and is encoded by the RELA gene in humans. It is composed of an N-terminal Rel homology domain, which contains the nuclear localization signal (NLS), and mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65. NF-kappaB p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation. Silencing of Rela induces tumor cell apoptosis in a murine Lewis lung carcinoma model, and RELA silencing in THP-1 monocytes decreases secreted levels of IL-1beta and TNF-alpha induced by LPS. Genome-wide deletion of Rela in mice is embryonic lethal. NF-kappaB p65 is overexpressed in the inflamed joints of patients with rheumatoid arthritis, and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-kappaB p65. Cayman's NF-kappaB (p65) NLS Polyclonal Antibody can be used for immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), IHC-paraffin (IHC-P), and Western blot (WB) applications. The antibody recognizes the NLS region of NF-kappaB to detect NF-kappaB at 65 kDa from human samples. It also recognizes the NLS region of NF-kappaB from chimpanzee, cow, gorilla, horse, monkey, and mouse samples.Synonyms: NF-kappaB3, Nuclear Factor-kappaB (p65) Nuclear Localization Signal, Transcription Factor p65. Immunogen: Peptide from the NF-kappaB (p65) NLS. Formulation: (Request formulation change), 100 µg of peptide affinity-purified polyclonal antibody. Storage Buffer: 200 µl PBS with 0.05% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, IF, IHC, IHC-P, WB. Origin: Animal/Rabbit.