Anti-Neuropeptide Y Receptor, Type 1 (NPY1R, NPYR, NPYY1, NPY1-R)

Neuropeptide Y receptor type 1(NPY1R) is a 44kD (unglycosylated) member of the G-protein coupled receptor 1 family. Human NPY1R is 384aa in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for neuropeptide Y and peptide YY. Applications: Suitable for use in Direct ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug x10e5 cells. Tested for use with MCF-7 human breast cancer cell line (1)., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.