Anti-Neuron Specific Enolase (NSE)

Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD), and gamma (46kD). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.