Anti-Neurofilament L (C-Term) Rabbit Monoclonal Antibody (Clone RM280)

Neurofilament L (NF-L) is one of four subunits that form NFs, which are type III intermediate filament proteins that enable axonal growth, maintain structure, and facilitate nerve conduction. NF-L is comprised of an N-terminal domain that regulates neurofilament assembly, a central alpha-helical rod region that mediates dimerization, and a glutamic acid-rich C-terminal tail that is subject to phosphorylation. It is abundantly expressed in myelinated axons in the central and peripheral nervous systems and localizes to the cytoplasm, where it associates with an NF-middle (NF-M) or -heavy (NF-H) subunit to form parallel coiled-coil heterodimers. These heterodimers then associate in an anti-parallel manner to form tetramers, which assemble into NFs. Cerebrospinal fluid (CSF) NF-L levels have been used as a marker of axonal injury in a variety of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), multiple sclerosis (MS), Parkinson's disease, and Alzheimer's disease. Mutations in NEFL, the gene encoding NF-L, have been found in patients with Charcot-Marie-Tooth disease type 2E (CMT2E), a neurological disease characterized by muscle weakness and atrophy. Cayman's Neurofilament L (C-Term) Rabbit Monoclonal Antibody (Clone RM280) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Neurofilament Light Polypeptide, NF-L. Immunogen: Peptide from the C-terminal region of human neurofilament L. Clone Designation: RM280. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC and WB. Cross Reactivity: (+) Neurofilament L.