Anti-Natriuretic Peptide Receptor A (NPR-A)

Natriuretic peptide receptor A/guanylate cyclase A (NPR-1), also called NPRA or GC-A, is a 120-140kD type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54-415), plus ATP to the intracellular kinase homology domain (aa 528-805) activates a cytoplasmic guanylate cyclase (aa 840-1023). NPR-1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR-1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR-1, human NPRB and human NPRC, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human NPR-1, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.