Anti-Natriuretic Peptide Receptor A (NPR-A)

Mouse Natriuretic Peptide Receptor-A (NPR-A), also known as NPR-1, ANP-R1 or guanlyl cyclase-A, is a 119 kD member of the guanylyl cyclase family of proteins. NPR-1 is a 1029 amino acid type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain and a 567 amino acid cytoplasmic region. The cytoplasmic region shows a tyrosine kinase and guanylyl cyclase catalytic domain. NPR-1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type n.p.) ANP binding induces bronchodilation and a reduction in blood pressure. Over the extracellular domain, mouse NPR-1 is 97% and 86% identical to rat and human NPR-1, respectively. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~10ng/lane under non-reducing and reducing conditions. , Direct ELISA: 0.5-1ug/ml. Detection limit is ~0.5ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.